HOOK3 anticorps (AA 14-198)

N° du produit ABIN6719547

Cet anticorps anti-HOOK3 est un anticorps Lapin Polyclonal détectant HOOK3 dans WB, ELISA, IF, IHC, FACS et ICC. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour HOOK3 anticorps (AA 14-198) (ABIN6719547)

Antigène: HOOK3 (Hook Homolog 3 (HOOK3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HOOK3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-HOOK3 anticorps

Épitope: AA 14-198

Fonction: Anti-HOOK3 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-HOOK3 Antibody Picoband® (ABIN6719547). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human HOOK3 recombinant protein (Position: E14-R198).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Urnavicius, L., Lau, C. K., Elshenawy, M. M., Morales-Rios, E., Motz, C., Yildiz, A., Carter, A. P. Cryo-EM shows how dynactin recruits two dyneins for faster movement. Nature 554: 202-206, 2018. 2. Walenta, J. H., Didier, A. J., Liu, X., Kramer, H. The Golgi-associated Hook3 protein is a member of a novel family of microtubule-binding proteins. J. Cell Biol. 152: 923-934, 2001.

Commentaires:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur HOOK3

Antigène: HOOK3 (Hook Homolog 3 (HOOK3))

Autre désignation: HOOK3

Sujet:

Synonyms: Protein Hook homolog 3, h-hook3, Hhk3, HOOK3

Tissue Specificity: Highly expressed in chromaffin cells of the adrenal medulla (at protein level). Detected in peripheral sympathetic ganglia (at protein level). Found in some paracrine cells in stomach and duodenum (at protein level).

Background: Protein Hook homolog 3 is a protein that in humans is encoded by the HOOK3 gene. Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.

Poids moléculaire: 83 kDa

ID gène: 84376

Pathways: Stem Cell Maintenance, Maintenance of Protein Location