CRB1 anticorps

N° du produit ABIN6719658

Cet anticorps anti-CRB1 est un anticorps Lapin Polyclonal détectant CRB1 dans WB, IHC et FACS. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour CRB1 anticorps (ABIN6719658)

Antigène: CRB1 (Crumbs Homolog 1 (CRB1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CRB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-CRB1 anticorps

Fonction: Anti-CRB1 Antibody Picoband®

Séquence: FRTRDANVII LHAEKEPEFL NISIQDSRLF FQLQ

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-CRB1 Antibody Picoband® (ABIN6719658). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human CRB1, which shares 87.9% and 84.8% amino acid (aa) sequence identity with mouse and rat CRB1, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: CRB1 crumbs homolog 1 (Drosophila)". 2. den Hollander, A. I., Davis, J., van der Velde-Visser, S. D., Zonneveld, M. N., Pierrottet, C. O., Koenekoop, R. K., Kellner, U., van den Born, L. I., Heckenlively, J. R., Hoyng, C. B., Handford, P. A., Roepman, R., Cremers, F. P. M. CRB1 mutation spectrum in inherited retinal dystrophies. Hum. Mutat. 24: 355-369, 2004.

Commentaires:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur CRB1

Antigène: CRB1 (Crumbs Homolog 1 (CRB1))

Autre désignation: CRB1

Sujet:

Synonyms: Protein crumbs homolog 1, CRB1

Tissue Specificity: Preferential expression in retina, also expressed in brain, testis, fetal brain and fetal eye.

Background: Crumbs homolog 1 is a protein that in humans is encoded by the CRB1 gene. This gene encodes a protein which is similar to the Drosophila crumbs protein and localizes to the inner segment of mammalian photoreceptors. In Drosophila crumbs localizes to the stalk of the fly photoreceptor and may be a component of the molecular scaffold that controls proper development of polarity in the eye. Mutations in this gene are associated with a severe form of retinitis pigmentosa, RP12, and with Leber congenital amaurosis. Alternate splicing results in multiple transcript variants, some protein coding and some non-protein coding.

Poids moléculaire: 154 kDa

ID gène: 23418

UniProt: P82279

Pathways: Signalisation Notch