His Tag anticorps (DyLight 649)

N° du produit ABIN6720637

N° du produit (Fournisseur): 200-343-382

Cet anticorps Souris Monoclonal détecte spécifiquement His Tag dans WB, ELISA, FM, DB et FLISA. Il présente une réactivité envers et a été mentionné dans 5+ publications.

Aperçu rapide pour His Tag anticorps (DyLight 649) (ABIN6720637)

Antigène: His Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp His Tag est conjugé à/à la DyLight 649

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Dot Blot (DB), FLISA

Clone: 33D10-D2-G8

Détail du produit anti-His Tag anticorps (DyLight 649)

N° du produit (Fournisseur): 200-343-382

Fournisseur: Rockland

Fonction: 6X His Tag Antibody DyLight™ 649

Réactivité croisée (Details): This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.

Purification: This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays.

Immunogène: This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Isotype: IgG1 kappa

Labeling Ratio: 2.5

Information d'application

Indications d'application:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000-1:25,000

Other: FLUORESCENT POLARIZATION: 2 - 20 nM

Commentaires:

Suggested Applications: Biochemical Assay, Microarray
Anti-6X HIS tagged Antibody conjugated to DyLight™649 has been tested by ELISA, dot blot, and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. Anti-6X HIS Antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Références pour anticorps anti-His Tag (DyLight 649) (ABIN6720637)

Daguer, Gonse, Shchukin, Farrera-Soler, Barluenga, Winssinger: "Dual Bcl-XL /Bcl-2 inhibitors discovered from DNA-encoded libraries using a fragment pairing strategy." dans: Bioorganic & medicinal chemistry, Vol. 44, pp. 116282, (2021) (PubMed).

Hunter, McIntosh, Shi, Sperberg, Funatogawa, Labanieh, Soon, Wastyk, Mehta, Carter, Hunter, Cochran: "An engineered ligand trap inhibits leukemia inhibitory factor as pancreatic cancer treatment strategy." dans: Communications biology, Vol. 4, Issue 1, pp. 452, (2021) (PubMed).

Mehta, Maddineni, Kelly, Lee, Hunter, Silberstein, Parra Sperberg, Miller, Rabe, Labanieh, Cochran: "An engineered antibody binds a distinct epitope and is a potent inhibitor of murine and human VISTA." dans: Scientific reports, Vol. 10, Issue 1, pp. 15171, (2020) (PubMed).

Kariolis, Miao, Diep, Nash, Olcina, Jiang, Jones, Kapur, Mathews, Koong, Rankin, Cochran, Giaccia: "Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies." dans: The Journal of clinical investigation, Vol. 127, Issue 1, pp. 183-198, (2017) (PubMed).

Kariolis, Miao, Jones, Kapur, Mathews, Giaccia, Cochran: "An engineered Axl 'decoy receptor' effectively silences the Gas6-Axl signaling axis." dans: Nature chemical biology, Vol. 10, Issue 11, pp. 977-83, (2014) (PubMed).

Détails sur His Tag

Antigène: His Tag

Sujet:

Synonyms: mouse anti-6X His Tag DyLight™ 649 conjugated Antibody, DyLight™ 649 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Background: Anti 6X HIS tagged Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. Anti-6X HIS Antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.