Fluorescein anticorps (DyLight 488)

N° du produit ABIN6720645

N° du produit (Fournisseur): 600-141-096

Cet anticorps anti-Fluorescein Polyclonal Chèvre conjugué à DyLight 488 (ABIN6720645) détecte spécifiquement Fluorescein dans WB, FLISA et FM.

Aperçu rapide pour Fluorescein anticorps (DyLight 488) (ABIN6720645)

Antigène: Fluorescein

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: DyLight 488

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Détail du produit

N° du produit (Fournisseur): 600-141-096

Fournisseur: Rockland

Fonction: Fluorescein Antibody Dylight™ 488 Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.

Purification: Fluorescein Antibody Dylight™ 488 Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogène: Fluorescein conjugated to Goat IgG

Isotype: IgG

Biological Activity Comment: 5.4

Information d'application

Indications d'application:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000 - 1:25,000

Other: User Optimized

Commentaires:

Anti-Fluorescein Antibody Dylight™ 488 Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détail du antigène

Antigène: Fluorescein

Classe de substances: Chemical

Sujet:

Synonyms: goat Anti-FITC antibody DyLight™ 488 conjugation, DyLight™ 488 conjugated goat anti-fluorescein antibody, DyLight™ 488 conjugated goat Anti-FITC antibody, goat anti-fluorescein antibody DyLight™ 488 conjugation

Background: Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Dylight™ antibody and reagent conjugates are specifically designed for protein detection methods that covers much of the visible spectrum and into the near-infrared region to visualize proteins in western blotting and other applications. Very low background fluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film. Dylight™conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging.