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DYKDDDDK Tag anticorps (DyLight 680)

Cet anticorps Souris Monoclonal détecte spécifiquement DYKDDDDK Tag dans WB, ELISA, FACS, FM et FLISA.
Rockland
N° du produit ABIN6720688
N° du produit (Fournisseur): 200-344-383
562,24 €
Plus frais de livraison 40,00 € et TVA
100 μg
Destination: France
Envoi sous 5 à 8 jours ouvrables

Aperçu rapide pour DYKDDDDK Tag anticorps (DyLight 680) (ABIN6720688)

Antigène

DYKDDDDK Tag

Reactivité

Veuillez nous consulter SVP

Hôte

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Souris

Clonalité

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Monoclonal

Conjugué

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Cet anticorp DYKDDDDK Tag est conjugé à/à la DyLight 680

Application

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Western Blotting (WB), ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM), FLISA

Clone

29E4-G7
  • N° du produit (Fournisseur)

    200-344-383

    Fournisseur

    Rockland

    Fonction

    DYKDDDDK Tag (Anti-FLAG®) Antibody Dylight™ 680 Conjugated

    Réactivité croisée (Details)

    The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

    Purification

    This antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays.

    Immunogène

    This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.

    Isotype

    IgG2a kappa

    Labeling Ratio

    1.9
  • Indications d'application

    FLISA_Dilution: >1:10,000

    ELISA_Dilution: 1:10,000 - 1:25,000

    Flow_Cytometry_Dilution: User Optimized

    IF_Microscopy_Dilution: >1:5,000

    Other: User Optimized

    Commentaires

    Anti-FLAG DyLight™680 has been tested by ELISA and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 100 μL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Date de péremption

    12 months
  • Antigène

    DYKDDDDK Tag

    Sujet

    Synonyms: mouse anti-FLAG™ tag DyLight™680 conjugation, Enterokinase Cleavage Site (ECS), DyLight™680 conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys

    Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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