His Tag anticorps (DyLight 488)

N° du produit ABIN6720712

N° du produit (Fournisseur): 200-341-382

Cet anticorps Souris Monoclonal détecte spécifiquement His Tag dans WB, ELISA, FM et FLISA. Il présente une réactivité envers .

Aperçu rapide pour His Tag anticorps (DyLight 488) (ABIN6720712)

Antigène: His Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp His Tag est conjugé à/à la DyLight 488

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), FLISA

Clone: 33D10-D2-G8

Détail du produit anti-His Tag anticorps (DyLight 488)

N° du produit (Fournisseur): 200-341-382

Fournisseur: Rockland

Fonction: 6X His Tag Antibody Dylight™ 488 Conjugated

Réactivité croisée (Details): This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.

Purification: This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays.

Immunogène: This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Isotype: IgG1 kappa

Labeling Ratio: 5.2

Information d'application

Indications d'application:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000-1:25,000

Other: FLUORESCENT POLARIZATION: 2 - 20 nMFRET: 2 - 20 nM

Commentaires:

Anti-6X His epitope tag DyLight™ 488 has been tested by ELISA and western blot. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur His Tag

Antigène: His Tag

Sujet:

Synonyms: mouse anti-6X His Tag DyLight™ 488 conjugated Antibody, DyLight™ 488 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.