His Tag anticorps (DyLight 549)
Aperçu rapide pour His Tag anticorps (DyLight 549) (ABIN6720714)
Antigène
Voir toutes His Tag AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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N° du produit (Fournisseur)
- 200-342-382
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Fournisseur
- Rockland
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Fonction
- 6X His Tag Antibody Dylight™ 549 Conjugated
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Réactivité croisée (Details)
- This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.
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Purification
- This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays.
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Immunogène
- This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
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Isotype
- IgG1 kappa
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Labeling Ratio
- 2.3
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Indications d'application
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FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000
Other: User Optimized
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Commentaires
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Anti-6X His Epitope Tag DyLight™549 has been tested by ELISA and Western Blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- His Tag
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Sujet
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Synonyms: mouse anti-6X His Tag DyLight™ 549 conjugated Antibody, DyLight™ 549 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody
Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Antigène
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