Recombinant Ubiquitin anticorps (Sortase,His tag,AVI tag)

N° du produit ABIN6731030

Cet anticorps anti-Ubiquitin Monoclonal Souris conjugué à Sortase,His tag,AVI tag (ABIN6731030) détecte spécifiquement Ubiquitin dans WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant Ubiquitin anticorps (Sortase,His tag,AVI tag) (ABIN6731030)

Antigène: Ubiquitin (Ub)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Ubiquitin est conjugé à/à la Sortase,His tag,AVI tag

Application: Western Blotting (WB)

Détail du produit anti-Ubiquitin anticorps (Sortase,His tag,AVI tag)

Fonction: AbFlex Ubiquitin antibody (rAb)

Marque: AbFlex®

Attributs du produit: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

Purification: Protein A Chromatography

Immunogène: This antibody was raised against bovine ubiquitin.

Isotype: IgG2a

Information d'application

Indications d'application: WB: 0.5 - 2 μg/mL DB: 2 μg/mL AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 39741.

Commentaires:

This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur Ubiquitin

Antigène: Ubiquitin (Ub)

Autre désignation: Ubiquitin

Sujet: AbFlex Ubiquitin antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Ubiquitin is an important signaling protein that is catalytically added to proteins on lysine residues to modify their function. Addition of multiple ubiquitin proteins in a string (poly-ubiquitylation) to proteins signals their destruction by the proteasome. Mono-ubiquitylation of proteins has a much different effect, more in keeping with post-translational modification of proteins by phosphorylation or acetylation. Mono-ubiquitylation is part of a number of signal transduction pathways, including those involving histone proteins and transcriptional regulation.

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway