Recombinant KDM4B anticorps (Sortase,His tag,AVI tag)

N° du produit ABIN6731039

Cet anticorps anti-KDM4B Monoclonal Souris conjugué à Sortase,His tag,AVI tag (ABIN6731039) détecte spécifiquement KDM4B dans WB et IP. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant KDM4B anticorps (Sortase,His tag,AVI tag) (ABIN6731039)

Antigène: KDM4B (Lysine (K)-Specific Demethylase 4B (KDM4B))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KDM4B est conjugé à/à la Sortase,His tag,AVI tag

Application: Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-KDM4B anticorps (Sortase,His tag,AVI tag)

Fonction: AbFlex JMJD2B / KDM4B antibody (rAb)

Marque: AbFlex®

Attributs du produit: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

Purification: Protein A Chromatography

Immunogène: This antibody was raised against full-length recombinant human lysine-specific demethylase 4B (KDM4B) protein.

Isotype: IgG2a

Information d'application

Indications d'application: IP: 4 μg per IP WB*: 0.05 - 2 μg/mL *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Commentaires:

This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, and 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur KDM4B

Antigène: KDM4B (Lysine (K)-Specific Demethylase 4B (KDM4B))

Autre désignation: JMJD2B / KDM4B

Sujet: AbFlex JMJD2B / KDM4B antibody was expressed in 293 cells and contains mouse immunoglobulin heavy and light chains. JMJD2B / KDM4B (JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, JHDM3B) is a histone demethylase that has been shown to demethylate histone H3 trimethylated at lysine 9. JMJD2B / KDM4B is physically associated with the MLL2 methyltransferase complex where it serves as a co-factor for ERa-mediated transcriptional activation.

Poids moléculaire: 130 kDa

NCBI Accession: NP_055830

Pathways: L'effet Warburg