HCN1 anticorps (AA 346-368)

N° du produit ABIN6735304

Cet anticorps Lapin Polyclonal détecte spécifiquement HCN1 dans WB et ELISA. Il présente une réactivité envers Humain, Rat, Souris et Lapin.

Aperçu rapide pour HCN1 anticorps (AA 346-368) (ABIN6735304)

Antigène: HCN1 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1))

Reactivité: Humain, Rat, Souris, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HCN1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-HCN1 anticorps

Épitope: AA 346-368

Specificité: The pore region of HCN1 (HCNP15-P) is very conserved among various HCNs. HCN1: mouse, rat, human, rabbit=100 % , HCN2: mouse, rat, human (86 %), HCN3: mouse (95 %), rat (90 %), HCN4: rat, human (95 %). The HCNP15-P sequence is also conserved in insect (Helothis virescens, 78 %), Drosophila (78 %) and Sea urchin (S. purpuratus, 69 %) HCNs. Therefore, antibodies to HCNP15-P should react with most HCNs and related sequences. Antibody reactivity in various species is not known. The HCNP15-P control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 μg control peptide per 1 μg of aff pure IgG or 1 μL antiserum) to confirm antibody specificity. This antibody may be useful in studying various HCNs and to examine the possibility of immunoblocking the pore region and impact on the functionality of HCNs proteins.

Purification: Immunoaffinity purified

Immunogène: A 23-aa peptide sequence corresponding to the pore region of human HCN1 corresponding to amino acids 346-368.

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (1:10000 - 1:100000), WB (1 - 10 μg/mL)

Usage: Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 μg/mL for affinity pure using ECL. ELISA: 1:10000-1:100000 using 50-100 ng of control peptide/well.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % BSA.

Conseil sur la manipulation: avoid freeze thaw cycles. Store undiluted.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Détails sur HCN1

Antigène: HCN1 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1))

Autre désignation: HCN1

Sujet: Name/Gene ID: HCN1
Subfamily: Potassium channel - cyclic nucleotide gated
Family: Ion Channel

Synonyms: HCN1, BCNG1, BCNG-1, HAC2, HAC-2

ID gène: 348980

UniProt: O60741

Pathways: Asymmetric Protein Localization