BRD4 anticorps (AA 651-700)

N° du produit ABIN214729

Cet anticorps Lapin Polyclonal détecte spécifiquement BRD4 dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Chien, Boeuf (Vache), Cobaye, Lapin, Cheval, Porc et Roussette (Chauve-souris).

Aperçu rapide pour BRD4 anticorps (AA 651-700) (ABIN214729)

Antigène: BRD4 (Bromodomain Containing 4 (BRD4))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Cobaye, Lapin, Cheval, Porc, Roussette (Chauve-souris)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BRD4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-BRD4 anticorps

Épitope: AA 651-700

Marque: IHC-plus™

Specificité: Human BRD4

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa651-700 of human BRD4 (O60885-2, NP_055114). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Lizard (100%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur BRD4

Antigène: BRD4 (Bromodomain Containing 4 (BRD4))

Autre désignation: BRD4

Sujet: Name/Gene ID: BRD4
Subfamily: BRD
Family: Protein Kinase

Synonyms: BRD4, Bromodomain containing 4, Chromosome-associated protein, MCAP, HUNK1, Protein HUNK1, Bromodomain-containing 4, HUNKI

ID gène: 23476

NCBI Accession: NP_055114

UniProt: O60885

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome