E2F4 anticorps (C-Term)

N° du produit ABIN6736723

L’anticorps Lapin Polyclonal anti-E2F4 a été validé pour WB, IHC et IHC (p). Il convient pour détecter E2F4 dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Cheval, Lapin, Poulet, Chien, Porc, Singe, Cobaye, Poisson zèbre (Danio rerio) et Roussette (Chauve-souris).

Aperçu rapide pour E2F4 anticorps (C-Term) (ABIN6736723)

Antigène: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Cheval, Lapin, Poulet, Chien, Porc, Singe, Cobaye, Poisson zèbre (Danio rerio), Roussette (Chauve-souris)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp E2F4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-E2F4 anticorps

Épitope: C-Term

Marque: IHC-plus™

Specificité: Human E2F4

Homologie: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Pig, Chicken, Zebrafish (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from C-Terminus of human E2F4 (Q16254, NP_001941). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Salmon, Zebrafish (100%), Xenopus, Stickleback (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur E2F4

Antigène: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Autre désignation: E2F4

Sujet: Name/Gene ID: E2F4

Synonyms: E2F4, E2F-4, p107/p130-binding protein, Transcription factor E2F4

ID gène: 1874

NCBI Accession: NP_001941

UniProt: Q16254

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases, Regulation of Cell Size