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alpha Tubulin anticorps (N-Term)

Cet anticorps anti-alpha Tubulin est un anticorps Souris Monoclonal détectant alpha Tubulin dans WB, ELISA, IHC, IF et IHC (p). Adapté pour Humain.
N° du produit ABIN364374

Aperçu rapide pour alpha Tubulin anticorps (N-Term) (ABIN364374)

Antigène

Voir toutes alpha Tubulin (TUBA1) Anticorps
alpha Tubulin (TUBA1)

Reactivité

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  • 79
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Humain

Hôte

  • 54
  • 54
  • 12
  • 2
  • 2
  • 1
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Souris

Clonalité

  • 74
  • 50
  • 2
Monoclonal

Conjugué

  • 80
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Cet anticorp alpha Tubulin est non-conjugé

Application

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Épitope

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    N-Term

    Marque

    IHC-plus™

    Specificité

    Recognizes alpha tubulin of various species. Recognizes a N-terminal structural domain of alpha tubulin. Reacts with peptides that correspond to position alpha (65-79) of Tubulin, band at 50kD.

    Purification

    Ammonium sulfate precipitation

    Immunogène

    Peptide corresponding to the position alpha (65-79) of Tubulin.

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG1
  • Indications d'application

    Approved: ELISA, IF (5 μg/mL), IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.4, 0.1 % sodium azide, 40 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • Antigène

    alpha Tubulin (TUBA1)

    Autre désignation

    TUBA1 / Tubulin Alpha 1

    Sujet

    Name/Gene ID: TUBA1

    Synonyms: TUBA1

    Pathways

    Dynamique des Microtubules
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