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EN1 anticorps (AA 252-301)

L’anticorps Lapin Polyclonal anti-EN1 a été validé pour WB. Il convient pour détecter EN1 dans des échantillons de Humain, Souris, Poulet, Rat, Cobaye, Boeuf (Vache), Singe et Porc.
N° du produit ABIN6741515

Aperçu rapide pour EN1 anticorps (AA 252-301) (ABIN6741515)

Antigène

Voir toutes EN1 Anticorps
EN1 (Engrailed Homeobox 1 (EN1))

Reactivité

  • 32
  • 20
  • 19
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Humain, Souris, Poulet, Rat, Cobaye, Boeuf (Vache), Singe, Porc

Hôte

  • 37
  • 1
Lapin

Clonalité

  • 34
  • 4
Polyclonal

Conjugué

  • 13
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp EN1 est non-conjugé

Application

  • 27
  • 16
  • 13
  • 13
  • 8
  • 7
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Épitope

    • 16
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    AA 252-301

    Specificité

    Human EN1

    Homologie

    Percent identity by BLAST analysis: Pig, Guinea pig (93%) Xenopus (92%) Rabbit (86%).

    Purification

    Immunoaffinity purified

    Immunogène

    Synthetic peptide located between aa252-301 of human EN1 (Q05925, NP_001417). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Bovine, Pig, Guinea pig, Chicken, Platypus, Lizard (100%), Gibbon, Galago, Elephant, Bat, Horse, Opossum (93%), Rabbit, Xenopus, Zebrafish (92%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Indications d'application

    Approved: WB (0.2 - 1 μg/mL)

    Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled water

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Conseil sur la manipulation

    Avoid repeat freeze-thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Antigène

    EN1 (Engrailed Homeobox 1 (EN1))

    Autre désignation

    EN1 / Engrailed

    Sujet

    Name/Gene ID: EN1

    Synonyms: EN1, Engrailed 1, Engrailed homolog 1, Homeobox protein en-1, Homeobox protein engrailed-1, Hu-En-1, Engrailed homeobox 1

    ID gène

    2019

    NCBI Accession

    NP_001417

    UniProt

    Q05925

    Pathways

    Dopaminergic Neurogenesis
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