SIN3A anticorps (N-Term)

N° du produit ABIN6741541

Cet anticorps Lapin Polyclonal détecte spécifiquement SIN3A dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Cheval, Chien, Porc, Roussette (Chauve-souris), Hamster et Singe.

Aperçu rapide pour SIN3A anticorps (N-Term) (ABIN6741541)

Antigène: SIN3A (SIN3 homolog A, transcription regulator (SIN3A))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Cheval, Chien, Porc, Roussette (Chauve-souris), Hamster, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SIN3A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-SIN3A anticorps

Épitope: N-Term

Marque: IHC-plus™

Specificité: Human SIN3A

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse (100%) Rabbit, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from N-Terminus of human SIN3A (Q96ST3, NP_056292). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig (100%), Rabbit, Opossum, Guinea pig (92%), Zebra finch, Platypus (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur SIN3A

Antigène: SIN3A (SIN3 homolog A, transcription regulator (SIN3A))

Autre désignation: SIN3A

Sujet: Name/Gene ID: SIN3A

Synonyms: SIN3A, DKFZP434K2235, KIAA0700

ID gène: 25942

NCBI Accession: NP_056292

UniProt: Q96ST3

Pathways: Activation of Innate immune Response, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha