PRDM5 anticorps (AA 36-85)

N° du produit ABIN6741548

Cet anticorps anti-PRDM5 est un anticorps Lapin Polyclonal détectant PRDM5 dans WB, IHC et IHC (p). Adapté pour Humain, Souris, Rat, Boeuf (Vache), Cobaye, Cheval, Poisson zèbre (Danio rerio), Lapin, Porc et Singe.

Aperçu rapide pour PRDM5 anticorps (AA 36-85) (ABIN6741548)

Antigène: PRDM5 (PR Domain Containing 5 (PRDM5))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Cobaye, Cheval, Poisson zèbre (Danio rerio), Lapin, Porc, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRDM5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PRDM5 anticorps

Épitope: AA 36-85

Marque: IHC-plus™

Specificité: Human PRDM5

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Dog, Chicken (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa36-85 of human PRDM5 (Q9NQX1, NP_061169). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Dog, Turkey, Zebra finch, Chicken (92%), Bat (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur PRDM5

Antigène: PRDM5 (PR Domain Containing 5 (PRDM5))

Autre désignation: PRDM5

Sujet: Name/Gene ID: PRDM5

Synonyms: PRDM5, BCS2, PFM2, PR domain containing 5, PR domain-containing protein 5

ID gène: 11107

NCBI Accession: NP_061169

UniProt: Q9NQX1