ST18 anticorps (AA 899-948)

N° du produit ABIN6741555

Cet anticorps Lapin Polyclonal détecte spécifiquement ST18 dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Lapin, Boeuf (Vache), Cobaye, Cheval, Porc, Roussette (Chauve-souris) et Singe.

Aperçu rapide pour ST18 anticorps (AA 899-948) (ABIN6741555)

Antigène: ST18 (ZNF387) (Zinc Finger Protein 387 (ZNF387))

Reactivité: Humain, Souris, Lapin, Boeuf (Vache), Cobaye, Cheval, Porc, Roussette (Chauve-souris), Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ST18 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ST18 anticorps

Épitope: AA 899-948

Marque: IHC-plus™

Specificité: Human ST18

Homologie: Percent identity by BLAST analysis: Human, Rabbit, Horse, Pig (100%) Rat, Dog, Chicken (92%) Mouse, Bovine, Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa899-948 of human ST18 (O60284, NP_055497). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Bat, Rabbit, Horse, Pig (100%), Galago, Rat, Dog, Turkey, Zebra finch, Chicken, Platypus (92%), Mouse, Bovine, Opossum, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur ST18

Antigène: ST18 (ZNF387) (Zinc Finger Protein 387 (ZNF387))

Autre désignation: ST18

Sujet: Name/Gene ID: ST18

Synonyms: ST18, KIAA0535, Zinc finger protein 387, ZC2HC10, ZNF387

ID gène: 9705

NCBI Accession: NP_055497

UniProt: O60284