ONECUT1 anticorps (C-Term)

N° du produit ABIN6741572

Cet anticorps Lapin Polyclonal détecte spécifiquement ONECUT1 dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Rat, Chien, Lapin, Porc, Cheval, Poisson zèbre (Danio rerio), Roussette (Chauve-souris) et Singe.

Aperçu rapide pour ONECUT1 anticorps (C-Term) (ABIN6741572)

Antigène: ONECUT1 (One Cut Homeobox 1 (ONECUT1))

Reactivité: Humain, Rat, Chien, Lapin, Porc, Cheval, Poisson zèbre (Danio rerio), Roussette (Chauve-souris), Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ONECUT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ONECUT1 anticorps

Épitope: C-Term

Marque: IHC-plus™

Specificité: Human ONECUT1

Homologie: Percent identity by BLAST analysis: Human, Rat, Dog, Rabbit, Pig (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from C-Terminus of human ONECUT1 (Q9UBC0, NP_004489). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Rat, Elephant, Dog, Bat, Rabbit, Horse, Pig, Zebra finch (100%), Bovine (92%), Mouse, Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur ONECUT1

Antigène: ONECUT1 (One Cut Homeobox 1 (ONECUT1))

Autre désignation: ONECUT1 / HNF6

Sujet: Name/Gene ID: ONECUT1

Synonyms: ONECUT1, Hepatocyte nuclear factor 6, HNF6, One cut domain family member 1, One cut homeobox 1, HNF-6, HNF6A

ID gène: 3175

NCBI Accession: NP_004489

UniProt: Q9UBC0