YAP1 anticorps (AA 351-400)

N° du produit ABIN462247

Cet anticorps Lapin Polyclonal détecte spécifiquement YAP1 dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Poisson zèbre (Danio rerio), Chien, Singe, Mouton, Poulet, Boeuf (Vache), Cheval, Porc, Lapin, Xenopus laevis et Roussette (Chauve-souris).

Aperçu rapide pour YAP1 anticorps (AA 351-400) (ABIN462247)

Antigène: YAP1 (Yes-Associated Protein 1 (YAP1))

Reactivité: Humain, Souris, Rat, Poisson zèbre (Danio rerio), Chien, Singe, Mouton, Poulet, Boeuf (Vache), Cheval, Porc, Lapin, Xenopus laevis, Roussette (Chauve-souris)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp YAP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-YAP1 anticorps

Épitope: AA 351-400

Marque: IHC-plus™

Specificité: Human YAP1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa351-400 of human YAP1 (P46937, NP_001123617). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur YAP1

Antigène: YAP1 (Yes-Associated Protein 1 (YAP1))

Autre désignation: YAP / YAP1

Sujet: Name/Gene ID: YAP1

Synonyms: YAP1, 65 kDa Yes-associated protein, YAP, Yes-associated protein beta, Yes-associated protein delta, Yorkie homolog, YAP2, Yes-associated protein 1, Yes-associated protein 2, YKI, YAP65

ID gène: 10413

NCBI Accession: NP_001123617

UniProt: P46937

Pathways: Signalisation MAPK, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha