CBLN4 anticorps (AA 125-174)
Aperçu rapide pour CBLN4 anticorps (AA 125-174) (ABIN6741612)
Antigène
Voir toutes CBLN4 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 125-174
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Marque
- IHC-plus™
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Specificité
- Human CBLN4
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Homologie
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).
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Purification
- Immunoaffinity purified
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Immunogène
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Synthetic peptide located between aa125-174 of human CBLN4 (Q9NTU7, NP_542184). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (100%), Salmon, Smelt (92%), Lizard, Seabass (85%).
Type of Immunogen: Synthetic peptide -
Isotype
- IgG
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Indications d'application
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Distilled Water.
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Concentration
- Lot specific
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Buffer
- Lyophilized from PBS with 2 % sucrose
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Conseil sur la manipulation
- Avoid repeat freeze-thaw cycles.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- CBLN4 (Cerebellin 4 Precursor (CBLN4))
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Autre désignation
- CBLN4 / Cerebellin 4
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Sujet
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Name/Gene ID: CBLN4
Synonyms: CBLN4, CBLNL1, Cerebellin precursor-like 1, Cerebellin-4, Cerebellin 4 precursor, Cerebellin-like glycoprotein 1, DJ885A10.1 -
ID gène
- 140689
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NCBI Accession
- NP_542184
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UniProt
- Q9NTU7
Antigène
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