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Ubiquilin 1 anticorps (AA 290-339)

L’anticorps Lapin Polyclonal anti-Ubiquilin 1 a été validé pour WB, IHC et IHC (p). Il convient pour détecter Ubiquilin 1 dans des échantillons de Humain, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin, Mouton et Singe.
N° du produit ABIN6741620

Aperçu rapide pour Ubiquilin 1 anticorps (AA 290-339) (ABIN6741620)

Antigène

Voir toutes Ubiquilin 1 (UBQLN1) Anticorps
Ubiquilin 1 (UBQLN1)

Reactivité

  • 55
  • 27
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Humain, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin, Mouton, Singe

Hôte

  • 48
  • 6
  • 2
Lapin

Clonalité

  • 51
  • 5
Polyclonal

Conjugué

  • 31
  • 5
  • 4
  • 4
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Ubiquilin 1 est non-conjugé

Application

  • 45
  • 35
  • 20
  • 8
  • 3
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Épitope

    • 8
    • 7
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 290-339

    Marque

    IHC-plus™

    Specificité

    Human UBQLN1

    Homologie

    Percent identity by BLAST analysis: Human, Sheep, Dog, Bovine, Rabbit, Horse (100%) Mouse, Rat, Chicken (85%).

    Purification

    Immunoaffinity purified

    Immunogène

    Synthetic peptide located between aa290-339 of human UBQLN1 (Q5T6J9, NP_038466). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Sheep, Elephant, Dog, Bovine, Rabbit, Horse, Guinea pig, Platypus (100%), Mouse, Rat, Panda, Bat (92%), Chicken (85%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Indications d'application

    Approved: IHC, IHC-P (5 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled Water.

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Conseil sur la manipulation

    Avoid repeat freeze-thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Antigène

    Ubiquilin 1 (UBQLN1)

    Autre désignation

    UBQLN1 / Ubiquilin

    Sujet

    Name/Gene ID: UBQLN1

    Synonyms: UBQLN1, DA41, HPLIC-1, PLIC-1, Ubiquilin, UBQN, XDRP1, DSK2, PLIC1, Ubiquilin 1, Ubiquilin-1

    ID gène

    29979

    NCBI Accession

    NP_038466

    UniProt

    Q9UMX0
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