HYAL1 anticorps (AA 36-85)

N° du produit ABIN490044

Cet anticorps anti-HYAL1 est un anticorps Lapin Polyclonal détectant HYAL1 dans WB, IHC et IHC (p). Adapté pour Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval et Roussette (Chauve-souris).

Aperçu rapide pour HYAL1 anticorps (AA 36-85) (ABIN490044)

Antigène: HYAL1 (Hyaluronidase-1 (HYAL1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval, Roussette (Chauve-souris)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HYAL1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-HYAL1 anticorps

Épitope: AA 36-85

Marque: IHC-plus™

Specificité: Human HYAL1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Horse, Guinea pig (100%) Rabbit, Pig (92%) Dog (85%) Bovine (83%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa36-85 of human HYAL1 (Q12794, NP_695014). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Mouse, Rat, Elephant, Bat, Horse, Guinea pig (100%), Galago, Marmoset, Rabbit, Pig (92%), Monkey, Dog (85%), Bovine (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur HYAL1

Antigène: HYAL1 (Hyaluronidase-1 (HYAL1))

Autre désignation: HYAL1

Sujet: Name/Gene ID: HYAL1

Synonyms: HYAL1, FUS2, Hyaluronidase-1, Hyaluronoglucosaminidase 1, LUCA1, Hyaluronoglucosaminidase-1, Plasma hyaluronidase, LuCa-1, Tumor suppressor LUCA-1, HYAL-1, Lung carcinoma protein 1

ID gène: 3373

NCBI Accession: NP_695014

UniProt: Q12794

Pathways: Glycosaminoglycan Metabolic Process