DDAH2 anticorps (N-Term)

N° du produit ABIN6744442

Cet anticorps anti-DDAH2 est un anticorps Lapin Polyclonal détectant DDAH2 dans WB, IHC et IHC (p). Adapté pour Humain, Souris, Rat, Chien, Cheval, Lapin et Roussette (Chauve-souris).

Aperçu rapide pour DDAH2 anticorps (N-Term) (ABIN6744442)

Antigène: DDAH2 (Dimethylarginine Dimethylaminohydrolase 2 (DDAH2))

Reactivité: Humain, Souris, Rat, Chien, Cheval, Lapin, Roussette (Chauve-souris)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DDAH2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-DDAH2 anticorps

Épitope: N-Term

Marque: IHC-plus™

Specificité: Human DDAH2

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from N-Terminus of human DDAH2 (O95865, NP_039268). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bat, Horse (100%), Monkey, Bovine (92%), Elephant (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur DDAH2

Antigène: DDAH2 (Dimethylarginine Dimethylaminohydrolase 2 (DDAH2))

Autre désignation: DDAH2 / DDAH

Sujet: Name/Gene ID: DDAH2

Synonyms: DDAH2, DDAH, DDAH-2, DDAHII, G6a, Alternative name: NG30, G6a, Unknown, Dimethylargininase-2, NG30, Protein G6a, S-phase protein

ID gène: 23564

NCBI Accession: NP_039268

UniProt: O95865