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MCTP1 anticorps (C-Term)

Cet anticorps Lapin Polyclonal détecte spécifiquement MCTP1 dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Cobaye, Cheval, Poisson zèbre (Danio rerio), Poulet, Singe et Porc.
N° du produit ABIN6744450

Aperçu rapide pour MCTP1 anticorps (C-Term) (ABIN6744450)

Antigène

MCTP1 (Multiple C2 Domains, Transmembrane 1 (MCTP1))

Reactivité

  • 7
  • 5
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Humain, Souris, Cobaye, Cheval, Poisson zèbre (Danio rerio), Poulet, Singe, Porc

Hôte

  • 5
  • 2
Lapin

Clonalité

  • 7
Polyclonal

Conjugué

  • 7
Cet anticorp MCTP1 est non-conjugé

Application

Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Épitope

    • 2
    • 2
    • 1
    • 1
    C-Term

    Marque

    IHC-plus™

    Specificité

    Human MCTP1

    Homologie

    Percent identity by BLAST analysis: Human, Mouse, Horse, Guinea pig, Chicken (100%).

    Purification

    Immunoaffinity purified

    Immunogène

    Synthetic peptide from C-Terminus of human MCTP1 (Q6DN14-2, NP_001002796). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Elephant, Horse, Pig, Opossum, Guinea pig, Chicken, Lizard (100%), Panda, Dog, Turkey (92%), Rat, Bat, Rabbit, Zebra finch, Platypus, Xenopus, Zebrafish (85%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Indications d'application

    Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled water

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Conseil sur la manipulation

    Avoid repeat freeze-thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Antigène

    MCTP1 (Multiple C2 Domains, Transmembrane 1 (MCTP1))

    Autre désignation

    MCTP1

    Sujet

    Name/Gene ID: MCTP1

    Synonyms: MCTP1

    ID gène

    79772

    NCBI Accession

    NP_001002796

    UniProt

    Q6DN14
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