HMGCS1 anticorps (AA 251-300)

N° du produit ABIN6744452

Cet anticorps anti-HMGCS1 est un anticorps Lapin Polyclonal détectant HMGCS1 dans WB, IHC et IHC (p). Adapté pour Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Lapin, Porc, Hamster et Xenopus laevis.

Aperçu rapide pour HMGCS1 anticorps (AA 251-300) (ABIN6744452)

Antigène: HMGCS1 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Lapin, Porc, Hamster, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HMGCS1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-HMGCS1 anticorps

Épitope: AA 251-300

Marque: IHC-plus™

Specificité: Human HMGCS1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Horse, Pig (100%) Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa251-300 of human HMGCS1 (Q01581, NP_002121). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Xenopus (100%), Marmoset, Bat, Guinea pig (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur HMGCS1

Antigène: HMGCS1 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1))

Autre désignation: HMGCS1 / HMG-CoA Synthase 1

Sujet: Name/Gene ID: HMGCS1

Synonyms: HMGCS1, HMG-CoA synthase, HMGCS

ID gène: 3157

NCBI Accession: NP_002121

UniProt: Q01581

Pathways: Regulation of Lipid Metabolism by PPARalpha