SH3BP2 anticorps (AA 432-481)

N° du produit ABIN6744458

Cet anticorps Lapin Polyclonal détecte spécifiquement SH3BP2 dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Singe et Boeuf (Vache).

Aperçu rapide pour SH3BP2 anticorps (AA 432-481) (ABIN6744458)

Antigène: SH3BP2 (SH3-Domain Binding Protein 2 (SH3BP2))

Reactivité: Humain, Souris, Rat, Singe, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SH3BP2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-SH3BP2 anticorps

Épitope: AA 432-481

Marque: IHC-plus™

Specificité: Human SH3BP2

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Rabbit, Guinea pig (92%) Bovine (84%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa432-481 of human SH3BP2 (P78314, NP_003014). Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Mouse, Rat, Opossum (100%), Elephant, Bat, Rabbit, Guinea pig (92%), Galago (85%), Bovine (84%), Zebra finch (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur SH3BP2

Antigène: SH3BP2 (SH3-Domain Binding Protein 2 (SH3BP2))

Autre désignation: SH3BP2

Sujet: Name/Gene ID: SH3BP2

Synonyms: SH3BP2, Abl-SH3 binding protein 2, 3BP-2, 3BP2, Cherubism, CRPM, RES4-23, SH3 domain-binding protein 2, TNFAIP3 interacting protein 2, CRBM, SH3-domain binding protein 2

ID gène: 6452

NCBI Accession: NP_003014

UniProt: P78314

Pathways: TCR Signaling