Septin 11 anticorps (N-Term)

N° du produit ABIN6744459

L’anticorps Lapin Polyclonal anti-Septin 11 a été validé pour WB, IHC et IHC (p). Il convient pour détecter Septin 11 dans des échantillons de Humain, Souris, Rat, Cheval, Boeuf (Vache), Cobaye, Lapin, Chien, Singe et Porc.

Aperçu rapide pour Septin 11 anticorps (N-Term) (ABIN6744459)

Antigène: Septin 11 (SEPT11)

Reactivité: Humain, Souris, Rat, Cheval, Boeuf (Vache), Cobaye, Lapin, Chien, Singe, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Septin 11 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Septin 11 anticorps

Épitope: N-Term

Marque: IHC-plus™

Specificité: Human SEPT11 / Septin 11

Homologie: Percent identity by BLAST analysis: Human, Orangutan, Monkey, Mouse, Rat, Bovine (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from N-Terminus of human SEPT11 (Q9NVA2, NP_060713). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur Septin 11

Antigène: Septin 11 (SEPT11)

Autre désignation: SEPT11 / Septin 11

Sujet: Name/Gene ID: SEPT11

Synonyms: SEPT11, Septin 11, Septin-11

ID gène: 55752

NCBI Accession: NP_060713

UniProt: Q9NVA2