CYR61 anticorps (AA 251-379)

N° du produit ABIN674499

Cet anticorps anti-CYR61 Polyclonal Lapin (ABIN674499) détecte spécifiquement CYR61 dans WB, IF, IHC (p) et IHC (fro). L’anticorps est réactif avec des échantillons de Humain. Validé indépendamment pour une utilisation en Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections).

Aperçu rapide pour CYR61 anticorps (AA 251-379) (ABIN674499)

Antigène: CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CYR61 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-CYR61 anticorps

Épitope: AA 251-379

Fonction: CCN1 Polyclonal Antibody

Réactivité croisée: Humain

Homologie: Mouse,Rat,Dog,Cow,Sheep,Horse

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human Cyr61

Isotype: IgG

Information d'application

Indications d'application: WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF()

Restrictions: For Research Use only

Independent Validation (IHC (fp))

Validation #100003 (Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections))

by: Memorial Sloan-Kettering Cancer Center, Parada lab

No.: #100003

Date: 15.10.2015

Antigène: CYR61

Application validée: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)

Contrôle positif: Tumor cell transplanted mouse brain was dissected

Conclusion: The staining pattern was as expected for Cyr61 as a secreted protein.

Validation Images

IF validation of CYR61 antibody ABIN674499 on mouse brain tumor sections. A. ABIN674499 diluted 1:100 (red), DAPI counterstaining (blue). B. Dilution factor 1:50. C. Dilution factor 1:100. D. Dilution factor 1:200.

Protocole

Anticorps primaire: ABIN674499

Anticorps secondaire: Donkey-anti-Rabbit-Cy3

Full Protocol:

• Tumor cell transplanted mouse brain was dissected, fixed in 4% PFA for overnight, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
• Immunostaining for Cryo Sections:
• Dry slides at 50-55°C for 15-30 min (or RT for 30min)
• Wash three times with PBS 5min (shake gently)
• Block with 10% Normal Donkey Serum (NDS) at RM for 1hour
• Incubate with primary antibody at 4°C overnight (Prepared in 2% NDS)
• Rabbit-anti-CYR61 antibody (ABIN674499) diluted 1:50-1:200
• Wash three times with PBS 5min (shake gently)
• Incubate with secondary antibody together with DAPI at RT for 1h (Prepared in 2% NDS), cover the chamber with foil
• Donkey-anti-Rabbit-Cy3
• DAPI 1:100
• Wash three times with PBS 5min (shake gently)
• Add three drops of water-based Immu-mount
• Cover with 24x60 cover glass
• Imaging
• Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.

Notes: ABIN674499 worked within the diution range 1:50 - 1:200

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur CYR61

Antigène: CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))

Autre désignation: Cyr61

Sujet:

Synonyms: CCN1, GIG1, IGFBP10, Protein CYR61, CCN family member 1, Cysteine-rich angiogenic inducer 61, Insulin-like growth factor-binding protein 10, IBP-10, IGF-binding protein 10, IGFBP-10, Protein GIG1, CYR61

Background: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.

ID gène: 3491

UniProt: O00622

Pathways: Positive Regulation of Endopeptidase Activity, Growth Factor Binding