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S100A8 anticorps

L’anticorps Chèvre Polyclonal anti-S100A8 a été validé pour WB, ELISA et ICC. Il convient pour détecter S100A8 dans des échantillons de Souris.
N° du produit ABIN6757634

Aperçu rapide pour S100A8 anticorps (ABIN6757634)

Antigène

Voir toutes S100A8 Anticorps
S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivité

  • 93
  • 48
  • 31
  • 12
  • 8
  • 8
  • 6
  • 4
  • 4
  • 4
  • 2
  • 2
  • 2
Souris

Hôte

  • 98
  • 34
  • 3
  • 3
  • 2
Chèvre

Clonalité

  • 103
  • 37
Polyclonal

Conjugué

  • 64
  • 23
  • 17
  • 6
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
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  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp S100A8 est non-conjugé

Application

  • 95
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  • 37
  • 31
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  • 18
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  • 6
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Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
  • Specificité

    Recognizes murine calgranulin A (S100 calcium-binding Protein A8) at ~10kD. In direct ELISAs and western blots, shows less than 5 % cross-reactivity with Recombinant, Murine S100A9, Recombinant, Murine S100A10 and Recombinant, Human S100B.

    Purification

    Immunoaffinity purified

    Immunogène

    E. coli-derived, Recombinant, Murine S100A8 Calgranulin A.

    Type of Immunogen: Recombinant protein

    Isotype

    IgG
  • Indications d'application

    Approved: ELISA (0.5 - 1 μg/mL), ICC, WB (0.1 - 0.2 μg/mL)

    Usage: Suitable for use in Western Blot, Immunocytochemistry and ELISA. Between each step, cells were washed with PBS containing 0.1 % BSA. Western Blot: 0.1-0.2 μg/mL. The detection limit is approximately 25 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. Direct ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect mouse S100A8. The detection limit for Recombinant, Murine S100A8 is approximately 0.2 ng/well.

    Commentaires

    Target Species of Antibody: Mouse

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS, 5 % trehalose, 40 % glycerol.

    Conseil sur la manipulation

    avoid freeze thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • Antigène

    S100A8 (S100 Calcium Binding Protein A8 (S100A8))

    Autre désignation

    S100A8 / MRP8

    Sujet

    Name/Gene ID: S100A8

    Synonyms: S100A8, 60B8AG, CFAG, CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, CGLA, L1Ag, NIF, p8, Protein S100-A8, MRP8, Urinary stone protein band A, CP-10, MA387, MRP-8

    ID gène

    6279

    UniProt

    P05109

    Pathways

    Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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