PCNA anticorps (AA 201-261)

N° du produit ABIN676598

Détail du produit anti-PCNA anticorps

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Épitope: AA 201-261

Reactivité: Humain, Souris, Rat, Poulet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Poulet, Humain, Souris, Rat

Homologie: Dog,Cow,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human PCNA

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA (PCNA Produits)

Synonymes: anticorps PCNAR, anticorps Pcna/cyclin, anticorps pcna, anticorps AO090003000846, anticorps 53/13, anticorps CG9193, anticorps DmPCNA, anticorps Dmel\\CG9193, anticorps MUS209, anticorps Mus209, anticorps PCNA, anticorps PCNA/mus209, anticorps Pcna, anticorps dPCNA, anticorps l(2)02448, anticorps l(2)56F, anticorps l(2)56Fa, anticorps l(2)s1534, anticorps cb16, anticorps fb36g03, anticorps wu:fa28e03, anticorps wu:fb36g03, anticorps pcna-A, anticorps proliferating cell nuclear antigen, anticorps DNA polymerase III sliding clamp, anticorps Proliferating cell nuclear antigen, anticorps Proliferating cell nuclear antigen-like, anticorps proliferating cell nuclear antigen L homeolog, anticorps proliferating cell nuclear antigen 1, anticorps PCNA, anticorps Pcna, anticorps pcna, anticorps MMP_RS08810, anticorps ANI_1_512014, anticorps AOR_1_1500154, anticorps MGC53867, anticorps pcna.L, anticorps pcna1, anticorps LOC106420145, anticorps pcn1

Sujet:

Synonyms: ATLD2, Proliferating cell nuclear antigen, PCNA, Cyclin

Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

ID gène: 5111

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy