Occludin anticorps (AA 431-522)

N° du produit ABIN687337

Cet validé indépendamment anticorps Lapin Polyclonal détecte spécifiquement Occludin dans WB, ELISA, FACS, IF (cc), IF (p) et IHC (p). Il présente une réactivité envers Humain, Souris et Porc et a été mentionné dans 5+ publications.

Aperçu rapide pour Occludin anticorps (AA 431-522) (ABIN687337)

Antigène: Occludin (OCLN)

Reactivité: Humain, Souris, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Occludin est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Occludin anticorps

Épitope: AA 431-522

Réactivité croisée: Humain, Souris, Porc

Homologie: Rat,Dog,Cow,Horse,Guinea Pig

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from mouse Occludin

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Independent Validation (IF)

Validation #029632 (Immunofluorescence)

by: Confocal Imaging Core, Beth Israel Deaconess Medical Center

No.: #029632

Date: 23.03.2014

Numéro du lot: 990698W

Application validée: Immunofluorescence

Contrôle positif: Mouse stomach tissue

Contrôle négative: Human fat tissue

Conclusion: Signal was detected in positive control sample and not in negative control sample.

Validation Images

Figure 1: Immunostaining (green) of mouse stomach tissue (A-C) and human fat tissue (D-F) with anti-OCLN (A, D), isotype control antibody (B, E), or with secondary alone (C, F). Tissues were counterstained with DAPI (blue).

Protocole

Anticorps primaire:

• Antibody: Occludin (OCLN)
• Catalog number: ABIN687337
• Lot number: 990698W

Anticorps secondaire:

• Antibody: Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alexa 647)

Full Protocol:

• Paraffin embedded positive and negative control tissue sections were deparaffinized and undergone antigen retrieval using 10 mM Sodium Citrate pH6 in a pressure cooker for 10 min.
• The tissue sections were incubated with 1 mg/mL Sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
• Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
• Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
• Tissue sections were rinsed three times in TBS for 5 min each at RT.
• Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
• Tissue sections were rinsed three times in TBS for 5 min each at RT.
• Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen)
• IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.

Notes:

• No challenges notes
• OCLN is recommended to staining of mouse stomach tissue according to this protocol.

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-Occludin (ABIN687337)

Sun, Liu, Xue, Wang: "Effects of insulin combined with idebenone on blood-brain barrier permeability in diabetic rats." dans: Journal of neuroscience research, Vol. 93, Issue 4, pp. 666-77, (2015) (PubMed).

Zhao, Qin, Han, Wang, Zhang, Liu: "?-Conglycinin reduces the tight junction occludin and ZO-1 expression in IPEC-J2." dans: International journal of molecular sciences, Vol. 15, Issue 2, pp. 1915-26, (2014) (PubMed).

Pan, Qin, Zhao, Wang, Liu, Che: "Effects of soybean agglutinin on mechanical barrier function and tight junction protein expression in intestinal epithelial cells from piglets." dans: International journal of molecular sciences, Vol. 14, Issue 11, pp. 21689-704, (2013) (PubMed).

Zhao, Qin, Sun, Che, Bao, Zhang: "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets." dans: International journal of molecular sciences, Vol. 12, Issue 12, pp. 8502-12, (2012) (PubMed).

Gu, Xue, Wei, Zhang, Li: "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." dans: Neuroscience letters, Vol. 493, Issue 3, pp. 140-4, (2011) (PubMed).

Détails sur Occludin

Antigène: Occludin (OCLN)

Autre désignation: Occludin

Sujet:

Synonyms: Ocl, AI53564, Occludin, Ocln

Background: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

ID gène: 18260

UniProt: Q61146

Pathways: Cell-Cell Junction Organization, Hepatitis C