Nucleolin anticorps (CF®488A)

N° du produit ABIN6934519

Cet anticorps anti-Nucleolin est un anticorps Souris Monoclonal détectant Nucleolin dans IF, FACS, ISt, StM et T. Adapté pour Humain.

Aperçu rapide pour Nucleolin anticorps (CF®488A) (ABIN6934519)

Antigène: Nucleolin (NCL)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nucleolin est conjugé à/à la CF®488A

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Staining Methods (StM), Test (T)

Clone: NCL-902

Détail du produit anti-Nucleolin anticorps (CF®488A)

Specificité: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Aucune reactivité croisée: Boeuf (Vache), Souris, Rat (Rattus)

Purification: Purified by Protein A/G

Immunogène: Recombinant human NCL protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: HeLa cells. Breast Cancer.

Known Application: Flow Cytometry (5 µL per test per one million cells in 0.1 mL or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Nucleolin

Antigène: Nucleolin (NCL)

Autre désignation: NCL

Poids moléculaire: 76kDa

ID gène: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization