CD31 anticorps (Extracellular Domain)

N° du produit ABIN6934749

L’anticorps Souris Monoclonal anti-CD31 a été validé pour WB et IHC (f). Il convient pour détecter CD31 dans des échantillons de Humain.

Aperçu rapide pour CD31 anticorps (Extracellular Domain) (ABIN6934749)

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD31 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 1A10

Détail du produit anti-CD31 anticorps

Épitope: Extracellular Domain

Specificité: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Réactivité croisée (Details): Human.

Immunogène: Prokaryotic recombinant fusion protein corresponding to a portion of the extracellular domain downstream of the signal sequence of the CD31 (PECAM-1)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1:50-1:100 for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: THP-1, Jurkat cells. Tonsil or Angiosarcoma.

Restrictions: For Research Use only

Stockage

Buffer: Tissue culture supernatant with 0.05% Azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD31

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Autre désignation: PECAM1

Sujet: EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA',CD31 / PECAM-1 (Endothelial Cell Marker)
Cellular localisation: Cell surface and cytoplasm of endothelial cells

Poids moléculaire: ~100kDa (endothelium) and ~130kDa (platelets)'

ID gène: 5175, 376675, 514412

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization