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ACTA1/ACTA2A/ACTG2 anticorps

Reactivité: Humain FACS, IF, IHC (f) Hôte: Souris Monoclonal HHF35 unconjugated
N° du produit ABIN6936350
  • Antigène Tous les produits ACTA1/ACTA2A/ACTG2
    ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))
    Reactivité
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 4
    Souris
    Clonalité
    • 4
    Monoclonal
    Conjugué
    • 4
    Cet anticorp ACTA1/ACTA2A/ACTG2 est non-conjugé
    Application
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Specificité
    This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
    Réactivité croisée (Details)
    Human. Rabbit. Cat. Dog. Mouse. Rat. Chicken.
    Purification
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogène
    SDS extract of human myocardium.
    Clone
    HHF35
    Isotype
    IgG1 kappa
  • Indications d'application

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: Muscle or sarcoma.

    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Agent conservateur
    Azide free
    Stock
    -20 °C,-80 °C
    Stockage commentaire
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Date de péremption
    24 months
  • Antigène
    ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))
    Abstract
    ACTA1/ACTA2A/ACTG2 Produits
    Sujet
    ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1,Actin, Muscle Specific (Muscle Cell Marker)
    Cellular localisation: Cytoplasmic
    Poids moléculaire
    Multiple
    ID gène
    58, 59, 70
    UniProt
    P62736, P68032, P68133
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