APC anticorps (AA 1-433)

N° du produit ABIN6936423

L’anticorps Souris Monoclonal anti-APC a été validé pour WB, ELISA, IF, FACS et Coat. Il convient pour détecter APC dans des échantillons de Humain.

Aperçu rapide pour APC anticorps (AA 1-433) (ABIN6936423)

Antigène: APC (Adenomatous Polyposis Coli (APC))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp APC est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

Clone: ALi 12-28

Détail du produit anti-APC anticorps

Épitope: AA 1-433

Specificité: The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner's syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. The cloning of the APC gene revealed a ubiquitously expressed protein, 2,843 amino acids in length, which is frequently mutated in patients suffering from FAP and GS. APC has been found to be associated with structural components of intracellular junctions. Functions as a tumor suppressor, promoting rapid degradation of CTNNB1 and is a negative regulator of Wnt signaling. Also plays a role in HGF-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

Aucune reactivité croisée: Souris

Réactivité croisée (Details): Human. Does not react with Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment of human APC protein (around aa 1-433)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: COLO 320DM whole cell lysate. HCT116 cells. Tonsil, placenta, breast or colon.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur APC

Antigène: APC (Adenomatous Polyposis Coli (APC))

Autre désignation: APC

Sujet: Adenomatous Polyposis Coli, CC1, Deleted in polyposis 2.5 (DP2.5), DP2, DP3, FAP, FPC, GS, Protein APC,APC / Adenomatous Polyposis Coli / FAP (Tumor Suppressor)
Cellular localisation: Cell membrane and cytoplasm.

Poids moléculaire: 66kDa

ID gène: 324, 158932

UniProt: P25054

Pathways: Signalisation WNT, Stem Cell Maintenance, Cell-Cell Junction Organization