ATG5 anticorps (AA 1-119)

N° du produit ABIN6936433

Cet anticorps anti-ATG5 est un anticorps Souris Monoclonal détectant ATG5 dans WB, ELISA, Coat et IHC (f). Adapté pour Humain.

Aperçu rapide pour ATG5 anticorps (AA 1-119) (ABIN6936433)

Antigène: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ATG5 est non-conjugé

Application: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: ATG5-2553

Détail du produit anti-ATG5 anticorps

Épitope: AA 1-119

Specificité: The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy, a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment of human ATG5 protein (around aa 1-119) (exact sequence is proprietary)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: ELISA (For coating use Ab at 1-5 μg/mL, order Ab without BSA), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: K562, U-87, A431, THP-1, PANC-1, Raji or HeLa cells. Ovary, Endometrium, Colon or Duodenum.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur ATG5

Antigène: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Autre désignation: ATG5

Sujet: APG5 autophagy 5 like (APG5L), Apoptosis-specific protein (ASP), ATG5, Autophagy related 5, hAPG5,ATG5 (Autophagy Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 32kDa

ID gène: 9474, 486063

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy