BrdU anticorps

N° du produit ABIN6936516

L’anticorps Souris Monoclonal anti-BrdU a été validé pour IHC, FACS, IF, Neut et StM. Il convient pour détecter BrdU dans des échantillons de .

Aperçu rapide pour BrdU anticorps (ABIN6936516)

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BrdU est non-conjugé

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Neutralization (Neut), Staining Methods (StM)

Clone: SPM166

Détail du produit anti-BrdU anticorps

Specificité: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Purification: Purified by Protein A/G

Immunogène: Bromodeoxyuridine (BrdU) conjugated to KLH

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Use Ab at 1 μg/mL for 30 minute at RT)[For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Buffer: 10 mM PBS without BSA and without Azide.

Agent conservateur: Azide free

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Date de péremption: 24 months

Détails sur BrdU

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Classe de substances: Chemical

Poids moléculaire: 307Da