BrdU anticorps

N° du produit ABIN6936516

L’anticorps anti-BrdU Monoclonal Souris est utilisé pour la détection de BrdU dans des échantillons de Différentes espèces. Il a été validé pour IHC, FACS et IF.

Aperçu rapide pour BrdU anticorps (ABIN6936516)

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Reactivité: Différentes espèces

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BrdU est non-conjugé

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: SPM166

Détail du produit anti-BrdU anticorps

Fonction: Bromodeoxyuridine (BrdU) Antibody

Specificité: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Bromodeoxyuridine (BrdU) conjugated to KLH

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Use Ab at 1 μg/mL for 30 minute at RT)[For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur BrdU

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Classe de substances: Chemical

Sujet:

Bromodeoxyuridine, BUdr

Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

Poids moléculaire: 307Da