C4A anticorps

N° du produit ABIN6936544

Cet anticorps anti-C4A est un anticorps Souris Monoclonal détectant C4A dans ELISA, Coat et IHC (f). Adapté pour Humain.

Aperçu rapide pour C4A anticorps (ABIN6936544)

Antigène: C4A (C4a) (Complement Fragment C4a (C4a))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp C4A est non-conjugé

Application: ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: C4D204

Détail du produit anti-C4A anticorps

Specificité: This MAb is specific to Complement 4d (C4d) and reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 Molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human Complement 4d protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: ELISA (For coating use Ab at 1-2 μg/mL order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1:200-1:400 for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Rejected Renal Transplant Tissue.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur C4A

Antigène: C4A (C4a) (Complement Fragment C4a (C4a))

Autre désignation: C4A

Sujet: Acidic Complement C4, Basic C4, C4-1, C4a Anaphylatoxin, C4A, C4A2, C4A3, C4A4, C4A6, C4b, C4B1, C4B12, C4d fragment, C4F, C4S, Complement C4 Gamma Chain, Complement Component 4A, Complement Component 4B,Complement 4d (C4d) (Acute Humoral Rejection Marker)
Cellular localisation: Intracytoplasmic vacuoles of endothelial cells and Secreted

Poids moléculaire: 192kDa (predicted)

ID gène: 720, 721, 534847

Pathways: Système du Complément