Caldesmon anticorps

N° du produit ABIN6936569

Détail du produit anti-Caldesmon anticorps

Antigène: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Caldesmon est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120'150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70'80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Crude human uterus extract

Clone: H-CALD

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (2-4 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Caldesmon

Antigène: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Autre désignation: CALD1 (CALD1 Produits)

Synonymes: anticorps CDM, anticorps H-CAD, anticorps HCAD, anticorps L-CAD, anticorps LCAD, anticorps NAG22, anticorps l-Cad, anticorps 4833423D12Rik, anticorps AI195384, anticorps AV071549, anticorps AW536160, anticorps C920027I18Rik, anticorps CALD1, anticorps Caldesmon, anticorps caldesmon 1, anticorps CALD1, anticorps Cald1

Sujet: CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22,Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 150kDa

ID gène: 800, 490203

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction