Caldesmon anticorps

N° du produit ABIN6936574

Cet anticorps anti-Caldesmon Polyclonal Lapin (ABIN6936574) détecte spécifiquement Caldesmon dans WB, IF et FACS. L’anticorps est réactif avec des échantillons de Humain et Rat.

Aperçu rapide pour Caldesmon anticorps (ABIN6936574)

Antigène: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Caldesmon est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-Caldesmon anticorps

Fonction: Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody

Specificité: Recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This antibody recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Réactivité croisée (Details): Human and Rat. Others not known.

Purification: 200ug/ml of Ab purified by Protein A/G.

Immunogène: Recombinant human CALD1 protein fragment

Isotype: IgG, kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistology (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Ovary, Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Caldesmon

Antigène: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Autre désignation: CALD1

Sujet:

CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22

Blood vessels in all tissues, ovary, smooth muscle or leiomyosarcoma, Uterus

Poids moléculaire: 120-150kDa

ID gène: 800, 490203

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction