Calnexin anticorps (AA 1-300)

N° du produit ABIN6936576

Détail du produit anti-Calnexin anticorps

Antigène: Calnexin (CANX)

Épitope: AA 1-300

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Calnexin est non-conjugé

Application: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: It recognizes a protein of 90 kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Aucune reactivité croisée: Rat (Rattus)

Réactivité croisée (Details): Human. Does not react with Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)

Clone: CANX-1541

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, MCF-7 or U2OS cells. Kidney or small intestine.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Calnexin

Antigène: Calnexin (CANX)

Autre désignation: CANX (CANX Produits)

Synonymes: anticorps CNX, anticorps IP90, anticorps P90, anticorps 1110069N15Rik, anticorps AI988026, anticorps Cnx, anticorps D11Ertd153e, anticorps canx, anticorps clgn, anticorps clnx, anticorps cnx, anticorps PP90, anticorps zgc:63524, anticorps wu:fe06b12, anticorps CANX, anticorps calnexin, anticorps CalNeXin, anticorps calnexin L homeolog, anticorps CANX, anticorps Canx, anticorps cnx-1, anticorps canx.L, anticorps canx, anticorps LOC100283947

Sujet: Calnexin, CANX, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88, P90,Calnexin (Endoplasmic Reticulum Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 90kDa

ID gène: 821, 567968

UniProt: P27824

Pathways: Signalisation MAPK, Thyroid Hormone Synthesis