Recombinant CD34 anticorps

N° du produit ABIN6936672

L’anticorps anti-CD34 Monoclonal Lapin est utilisé pour la détection de CD34 dans des échantillons de Humain et Rat. Il a été validé pour FACS et IHC.

Aperçu rapide pour Recombinant CD34 anticorps (ABIN6936672)

Antigène: CD34

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp CD34 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: HPCA1-1806R

Détail du produit anti-CD34 anticorps

Fonction: Recombinant CD34 (Hematopoietic Stem Cell & Endothelial Marker) Antibody

Specificité: This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85 % of angiosarcoma and Kaposi's sarcoma, but shows low specificity.

Réactivité croisée (Details): Human. Rat.

Purification: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogène: Recombinant full-length human HPCA1 protein

Isotype: IgG, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat, KG-1 cells. Human tonsil or angiosarcoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD34

Antigène: CD34

Autre désignation: CD34

Sujet:

Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Jurkat, KG-1 cells. Human tonsil or angiosarcoma.

Poids moléculaire: 90-110kDa

ID gène: 947, 374990

UniProt: P28906