CD40 anticorps

N° du produit ABIN6936709

Cet anticorps anti-CD40 est un anticorps Souris Monoclonal détectant CD40 dans FACS, IF, IHC (f) et DNA Mic. Adapté pour Humain.

Aperçu rapide pour CD40 anticorps (ABIN6936709)

Antigène: CD40

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD40 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), DNA Microscopy (DNA Mic)

Clone: C40-1605

Détail du produit anti-CD40 anticorps

Specificité: CD40 is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is expressed on B-lymphocytes, follicular dendritic cells, bone marrow-derived dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zones of secondary lymphoid organs.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human CD40 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Immunofluorescence (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: U2OS, L-428 cells. Tonsil, Spleen or Thymus.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CD40

Antigène: CD40

Autre désignation: CD40

Sujet: B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, GP39, HIGM1, IGM, IMD3, p50, TBAM, TNF receptor superfamily member 5, TNFRSF5, TRAP,CD40 / TNFRSF5 / CD40L-Receptor
Cellular localisation: Cell Surface and Secreted

Poids moléculaire: 43kDa

ID gène: 958, 472860

UniProt: P25942

Pathways: Signalisation NF-kappaB, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints