CD40 Ligand anticorps (AA 108-261)
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- Antigène Voir toutes CD40 Ligand (CD40LG) Anticorps
- CD40 Ligand (CD40LG)
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Épitope
- AA 108-261
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD40 Ligand est non-conjugé
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificité
- CD40LG expression is mainly confined to the CD4-positive-T-cell subset. Its expression is induced shortly after T-cell activation and represents an early activation marker of T lymphocytes. CD40 is constitutively expressed mainly on B cells, macrophages, and dendritic cells. The CD40-CD40L pathway has been shown to play multiple functional roles in the healthy immune system. It enhances the antigen-specific T-cell response through the activation of dendritic cells and the induction of interleukin-12 production. For example, engagement of CD40 on endothelial cells by activated T cells expressing CD40L leads to upregulation of adhesion molecules such as ICAM-1, VCAM-1, and E-selectin. Activation of APC by CD40-CD40L interaction induces the production of inflammatory cytokines, chemokines, NO, and metalloproteinases. Interaction of CD4-positiveCD40LG-positiveT cells with CD40 on B cells leads to B-cell differentiation, proliferation, immunoglobulin (Ig) isotype switching, and formation of memory B cells.
- Réactivité croisée (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogène
- Recombinant fragment of human CD40LG protein (around aa 108-261) (exact sequence is proprietary)
- Clone
- CD40LG-2763
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD40LG Anticorps primaire
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- Indications d'application
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Known_Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat cells. Human tonsil, spleen or thymus tissue (IHC).
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Agent conservateur
- Azide free
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Date de péremption
- 24 months
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- Antigène
- CD40 Ligand (CD40LG)
- Autre désignation
- CD40LG (CD40LG Produits)
- Synonymes
- anticorps CD154, anticorps cd40l, anticorps CD40L, anticorps HIGM1, anticorps IGM, anticorps IMD3, anticorps T-BAM, anticorps TNFSF5, anticorps TRAP, anticorps gp39, anticorps hCD40L, anticorps CD40-L, anticorps Cd40l, anticorps Ly-62, anticorps Ly62, anticorps Tnfsf5, anticorps CD40 ligand, anticorps TNF superfamily member 5, anticorps CD40LG, anticorps cd40l, anticorps Cd40lg
- Sujet
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CD154, gp39, hCD40L, HIGM1, IGM, IMD3, T B cell activating molecule, T BAM, T-cell antigen Gp39, TNF-related activation protein,TrAP, Tumor necrosis factor ligand superfamily member 5 (TNFSF5),CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes)
Cellular localisation: Cell surface. Secreted. - Poids moléculaire
- 36kDa
- ID gène
- 959, 592244
- UniProt
- P29965
- Pathways
- Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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