CDC20 anticorps
-
- Antigène Voir toutes CDC20 Anticorps
- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
-
Reactivité
- Humain
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp CDC20 est non-conjugé
-
Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificité
- Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
- Réactivité croisée (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogène
- Urea-denatured His6 Cdc20 human recombinant protein
- Clone
- AR12
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CDC20 Anticorps primaire
-
-
- Indications d'application
-
Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Ramos or HeLa cells. Tonsil or gastric carcinoma.
- Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Agent conservateur
- Azide free
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Date de péremption
- 24 months
-
- Antigène
- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
- Autre désignation
- CDC20 (CDC20 Produits)
- Synonymes
- anticorps X-FZY, anticorps ba276h19.3, anticorps cdc20a, anticorps fizzy1, anticorps p55cdc, anticorps CDC20A, anticorps bA276H19.3, anticorps p55CDC, anticorps 2310042N09Rik, anticorps C87100, anticorps cell division cycle 20, anticorps cell division cycle 20 L homeolog, anticorps cell division cycle 20 homolog (S. cerevisiae), anticorps cdc20, anticorps CDC20, anticorps cdc20.L, anticorps Cdc20
- Sujet
-
CDC20, CDC20A, p55CDC, P55CDC-LSB,Cdc20 (Cell Division Cycle Protein 20)
Cellular localisation: Cytoplasmic - Poids moléculaire
- 55kDa
- ID gène
- 991, 524947
- UniProt
- Q12834
-