Chromogranin A anticorps

N° du produit ABIN6936974

Cet anticorps Souris Monoclonal détecte spécifiquement Chromogranin A dans IF, FACS et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour Chromogranin A anticorps (ABIN6936974)

Antigène: Chromogranin A (CHGA)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Chromogranin A est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: SPM339

Détail du produit anti-Chromogranin A anticorps

Specificité: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

Aucune reactivité croisée: Cobaye, Lapin, Mouton

Réactivité croisée (Details): Human. Monkey. Pig. Mouse. Rat. Does not react with Guinea Pig, Sheep or Rabbit.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Human pheochromocytoma

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: PC12 cells. Adrenal gland, bowel, thyroid, pancreas or pheochromocytoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Chromogranin A

Antigène: Chromogranin A (CHGA)

Autre désignation: CHGA

Sujet: Beta-Granin, CGA, CHGA, Chromogranin A Parathyroid Secretory Protein 1, ER-37, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I, Vasostatin I or II,Chromogranin A (Neuroendocrine Marker)
Cellular localisation: Finely granular cytoplasmic

Poids moléculaire: 68-75kDa

ID gène: 1113, 150793

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling