ENO2/NSE anticorps (AA 416-433)

N° du produit ABIN6937150

Cet anticorps anti-ENO2/NSE est un anticorps Souris Monoclonal détectant ENO2/NSE dans WB, IF, FACS et IHC (f). Adapté pour Humain.

Aperçu rapide pour ENO2/NSE anticorps (AA 416-433) (ABIN6937150)

Antigène: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ENO2/NSE est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: SPM347

Détail du produit anti-ENO2/NSE anticorps

Épitope: AA 416-433

Specificité: Recognizes a protein of about 50 kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: A synthetic peptide of human NSE gamma (around aa416-433, exact sequence is proprietary)

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur ENO2/NSE

Antigène: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Autre désignation: ENO2

Sujet: 2-phospho-D-glycerate hydrolyase, ENO2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE,NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: ~50kDa

ID gène: 2026, 511915

UniProt: P09104