FAF1 anticorps

N° du produit ABIN6937211

Cet anticorps Souris Monoclonal détecte spécifiquement FAF1 dans WB et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour FAF1 anticorps (ABIN6937211)

Antigène: FAF1 (Fas (TNFRSF6) Associated Factor 1 (FAF1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FAF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CPTC-FAF1-2

Détail du produit anti-FAF1 anticorps

Specificité: In contrast to growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis. Cellular response to FAS-L and TNF is mediated by structurally related receptors containing a conserved 'death domain' and belonging to the TNF receptor superfamily. TRADD, FADD and RIP are FAS/TNF-RI interacting proteins that contain a death domain homologous region (DDH). TRADD (TNF-RI-associated death domain) and FADD (FAS-associated death domain) associate with the death domains of both FAS and TNF-RI via their DDH regions, while RIP associates exclusively with FAS. An additional FAS interacting protein designated FAF1, for FAS-associated protein factor-1, binds with the cytoplasmic tail of wildtype but not LPR mutant FAS. When overexpressed in cells, FAF1 enhances the efficiency of FAS-mediated apoptosis. In contrast to TRADD, FADD and RIP, FAF1 lacks a DDH and cannot induce apoptosis independently of FAS activation.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human full-length FAF1 protein

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HEK293, MCF-7, HeLa whole cell lysate. Human testis, prostate, breast carcinoma or colon carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur FAF1

Antigène: FAF1 (Fas (TNFRSF6) Associated Factor 1 (FAF1))

Autre désignation: FAF1

Sujet: CGI-03, FAS-associated factor 1, HFAF1s, UBX domain-containing protein 12, UBX domain-containing protein 3A,Fas (TNFRSF6) associated factor 1
Cellular localisation: Nucleus.

Poids moléculaire: 75-80kDa

ID gène: 11124, 530402

UniProt: Q9UNN5

Pathways: Maintenance of Protein Location