Recombinant HSPD1 anticorps

N° du produit ABIN6937451

Cet anticorps Lapin Monoclonal détecte spécifiquement HSPD1 dans WB, IHC (f) et ISt. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant HSPD1 anticorps (ABIN6937451)

Antigène: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp HSPD1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)

Clone: HSPD1-2206R

Détail du produit anti-HSPD1 anticorps

Specificité: Recognizes a 60 kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone HSPD1/2206R, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.

Réactivité croisée (Details): Human, Mouse, Rat, Hamster, Sheep, Rabbit, Cow, Dog, Pig, Monkey and Chicken.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human full-length HSP60 protein

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.

Restrictions: For Research Use only

Stockage

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur HSPD1

Antigène: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Autre désignation: HSPD1

Sujet: 60 kDa chaperonin, 60 kDa heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (SPG13),HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)
Cellular localisation: Mitochondria in cytoplasm

Poids moléculaire: 60kDa

ID gène: 3329, 595053

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity